We found that the regulated genes by miR-10b were mainly enriched in PI3K-Akt signaling pathway, apoptosis, colorectal cancer, central carbon metabolism in cancer, pancreatic cancer, estrogen signaling pathway, TNF signaling pathway, sphingolipid signaling pathway, neurotrophin signaling pathway, AMPK signaling pathway and FoxO signaling pathway. The gene discussed is TNF; the disease is colorectal cancer.