In order to further determine the effects of ASP on MAPK and PI3K signaling pathways in HCC cells (SK-Hep1 and Hep-3B) under hypoxic conditions, we examined the protein levels of AKT, p-AKT, mTOR, p-mTOR, ERK and p-ERK after ASP treatment for 24 h under hypoxic conditions in SK-Hep1 and Hep-3B cells. The gene discussed is AKT1; the disease is hepatocellular carcinoma.