INS and obesity due to melanocortin 4 receptor deficiency: The analysis indicated that the most significantly enriched gene sets were positively correlated with the obesity group, and included pathogenic Escherichia coli infection, the complement and coagulation cascades, the toll-like receptor signaling pathway, the chemokine signaling pathway, and cytokine-receptor interactions (Figures 3A,B), while top gene sets negatively correlated with obesity were involved in fatty acid metabolism, the insulin signaling pathway, adipocytokine signaling pathway, the citric acid cycle, and the ribosome.