In this study, six hub genes (including CEP55, DEPDC1, CLSPN, KIF23, MYBL2, and RACGAP1) were identified after comprehensive bioinformatic analysis in HCC cohort from TCGA, and were all overexpressed in HCC patients, which was validated in mRNA and protein expressed level based on various HCC cohorts from Oncomine and experimental data in HCC cell lines and normal liver cell line by qPCR method. Here, DEPDC1 is linked to hepatocellular carcinoma.