For that, SCs were incubated with WT TTR as a control, I68L TTR (a cardiomyopathy mutation), T119M TTR (a non-pathological mutation) and I84S (a mutation that leads to carpal tunnel syndrome and affects heart and eye) for 24 hours and assessed the expression levels of several chemokines by real-time PCR, as before. The gene discussed is TTR; the disease is carpal tunnel syndrome.