As shown in Figure 7, there were 6 related genes significantly down-regulated in the NTDs group, compared with the control group (P < 0.01), which included Intraflagellar Transport 80 (Ift80), Kirsten rat sarcoma viral oncogene homolog (Kras), McKusick-Kaufman Syndrome (Mkks), Polycystic Kidney and Hepatic Disease 1 (Pkhd1), Protein Kinase C Alpha (Prkca) and Smoothened (Smo). The gene discussed is MKKS; the disease is McKusick-Kaufman syndrome.