AGT and hypertensive heart disease: In order to investigate mechanisms linking NPR-C signaling, diastolic dysfunction, and HFpEF, we used an established model of hypertensive heart disease in which mice are chronically administered angiotensin II (Ang II) (3 mg/kg per day for 3 weeks by miniosmotic pump) (Frohlich et al., 1992; Houser et al., 2012; Mackasey et al., 2018).