In present studies bicyclol were used in healthy rat, NAFLD rat and T2DM–NAFLD rat, compared with healthy-rat and T2DM–NAFLD rat, our work suggested that bicyclol might effectively improve T2DM–NAFLD related symptoms include improve the hepatic insulin signal, suppress enhancement of the hepatic gluconeogenesis and reduce the liver injury through an anti-inflammatory mechanism. Here, INS is linked to metabolic dysfunction-associated steatotic liver disease.