TNF and neoplasm: To explore the cytokine production [IFN-γ, TNF-α and granzyme B (GzmB)] and antigen (Ag)-specificity of treatment-induced CD8+ Tc responses in the tumor microenvironment, we conducted in vitro recall experiments using the tumor-infiltrating T-cell samples isolated from above YUMM3.3 and B16F10 tumors (Suppl.Fig. 9).