To further evaluate the regulatory mechanism of miR-335 in ovarian tumor cell lines, we found that wt-XIST 3′UTR luciferase report vector (wt-XIST) and mut-XIST 3′UTR luciferase report vector (mut-XIST) by successively mutating the predicted miR-335 binding site in XIST 3′UTR (Fig. 4A). The gene discussed is XIST; the disease is ovarian neoplasm.