In addition, we observed that Cluster of differentiation 40 (CD40, p = 0.017), C–C motif chemokine 16 (CCL16, p = 0.019) and IL-6 (p = 0.021) were highly secreted from PDS MCF7-cultures from patients with lymph node metastasis. This evidence concerns the gene CD40 and metastatic malignant neoplasm in the lymph nodes.