Similarly, by plotting the ROC curve for discrimination between cancer and non-cancer groups (benign and healthy) (Fig. 1), a significant difference was reported (P< 0.0001) between the three groups as the frequency of the positivity rate (> cutoff point) was superior for breast cancer cases at 91.3%, 92.9%, and 91.8% for miRNA-373, VEGF, and cyclin D1, respectively. Here, CCND1 is linked to breast cancer.