In contrast, slight red fluorescence representing JCPyV VLP-SPB-TAMRA was detected after addition to HT-1197 (Fig. 3C) and HT-1376 (Fig. 3D) bladder cancer cells at low concentrations (1 μg, 3 μg), and obvious red fluorescence was observed in both cell lines when the concentration of JCPyV VLP-SPB-TAMRA was increased to 10 μg (Fig. 3C,D). The gene discussed is SFTPB; the disease is urinary bladder cancer.