CD8A and neoplasm: On day 6, some mice received a limited number of 1×105 naïve CD8+ T cells isolated from 1G4 mice by negative selection and subsequently were infected on next day either with no vaccinia, 1×106 pfu of rVV NY-ESO-1 9V or rVV SIINFEKL by intravenous administration to model an NY-ESO-1 directed tumor vaccine.