WNT1 and neoplasm: Despite this, limiting dilution studies in nu/nu mice on doxycycline using YFP+CD45−DAPI− tumor cells isolated from syngeneic pairs of orthotopic primary tumors and residual lesions derived from individual donor tumors arising in MTB;TetO-Wnt1;TTC;rYFP mice revealed that residual Wnt1 tumor cells exhibited lower TIC frequencies than primary tumor cells (Additional file 10: Table S4).