Besides, flow cytometry was performed to examine the effect of YTHDF1 on the HCC cell cycle process and it showed that the inhibition of YTHDF1 in Huh7 and MHCC-97H cells increased the percentage of G0/G1 cells (Fig. 3F), suggesting that the downregulation of YTHDF1 induced cell cycle arrest of HCC cells. The gene discussed is YTHDF1; the disease is hepatocellular carcinoma.