AIM2 and neoplasm: Therefore, to evaluate the involvement of AIM2 in tumor progression, we performed a ROC analysis to calculate AIM2 cut-off, which was defined as 907 μm2 (according to the ImageJ analysis of immunohistochemical pictures) and as defined by values of sensitivity and specificity of 75% (40,93% to 95,56% at a confidence interval (CI) of 95%).