As shown in Figure 2G, 13 pathways upregulated in normal tissue Treg were downregulated in Treg from malignant tissues including cardiac hypertrophy signaling (Enhanced), TREM-1 signaling, production of nitric oxide and reactive oxygen species in macrophages, IL-15 signaling, IL-8 signaling, colorectal cancer metastasis signaling, role of pattern recognition receptors in recognition of bacteria and viruses, neuroinflammation signaling pathway, NF-kB signaling, B cell receptor signaling, Toll-like receptor signaling, inflammasome pathway, and STAT3 pathway. The gene discussed is NFKB1; the disease is colorectal cancer.