Our results have demonstrated that normal tissue Treg and non-tumor diseased tissue Treg have quite low CTLA-4 dependent but high PD-1 dependent transcriptomic changes, suggesting that CTLA-4 does not, but PD-1 does, play significant Foxp3-non-overlapped (131) roles in promoting Treg upregulated genes in normal and non-tumor diseased tissue Treg; and tumor spleen Treg and tumor Treg have certain transcriptomic changes in CTLA-4, and PD-1, non-collaboration manners with innate immune dominant pathways. Here, CTLA4 is linked to neoplasm.