Our study aimed to establish a septic myocardial injury model to study whether APN preconditioning reduces myocardial injury in sepsis, to explore whether APN can protect myocardial injury through the Cx43 and PI3K/AKT signalling pathways and to investigate the relationship between Cx43 and PI3K/AKT signalling. The gene discussed is AKT1; the disease is Sepsis.