INS and Insulin resistance: Consistent with the improvement in systemic insulin resistance and in iWAT insulin sensitivity that we observed in BATiPLIN5 mice, the livers of these mice showed significantly less steatosis than Control mice after 8 weeks of HFD, as assessed by oil red O staining (Fig. 5f), H&E staining (Supplementary Fig. 9), and biochemical TAG extraction and quantification (Fig. 5g).