LAMP2 and Autoimmunity: To reduce potential autoimmunity, we use male mice for isolating neutrophils for this experiment, as male’s immunity is less sensitive than female’s.32 Next, we stained LAMP2 (green) and LD (red) in the tissue, and found that after PAO1 infection, increased colocalization between LAMP2 and LDs, whereas deleting neutrophils reduced the colocalization.