We chose one or more leukodystrophy patient representative of each disease category, and investigated expression of claudin-5 (Fig. 1), ZO-1 (Fig. 2), laminin (Fig. 1), PDGFRβ (Fig. 1), α-dystroglycan (Fig. 3) and AQP4 (Fig. 4) together with UEA I and/or GFAP. This evidence concerns the gene CLDN5 and leukodystrophy.