TJP1 and infection: BCECs from the OB (Figs. 3 and 4a–h) and cortical regions (Figs. 3 and 4i–p) of the brain from weanling (Figs. 3 and 4a–d and i–l) or adult mice (Fig. 3 and 4e–h and m–p) were cultured, kept uninfected as mock samples, and infected separately with 0.1, 1, and 10 MOI of LACV and then analyzed for infection using markers against LACV (green) to detect virus and ZO1 (red) to look at endothelial cell structure in the culture.