Our study found that MMR status was not related to 253 lymph node metastasis, however, the immunohistochemical staining intensities of MSH6 and MLH1 in the 253 lymph node metastasis group were weaker than those in the non-metastatic group (68.0 ± 26.1% vs. 78.9 ± 13.1%, P = 0.013; 56.5 ± 24.3% vs. 72.5 ± 15.6%, P = 0.002). The gene discussed is MSH6; the disease is metastatic malignant neoplasm in the lymph nodes.