This study was intended to explore the mechanism of lncRNA HOTAIR in regulating PPARα, to provide an in-depth description of the role of HOTAIR in the malignant progression of glioma by in vivo animal experiments and in vitro cell line experiments and to verify the therapeutic effect of the combination of si-HOTAIR and the PPARα agonist fenofibrate on glioma cells. This evidence concerns the gene PPARA and glioma.