Differences in TSPO expression in the MI model could be detected and quantified with [18F]FEDAC PET–CT, with normal-to-ischemic uptake ratios from 10.47 ± 3.03 (1.5 min) to 3.92 ± 1.12 (27.5 min) (P = 0.025), enabling the non-invasive imaging of myocardial injury in the acute phase as early as 30 min after injection. Here, TSPO is linked to myocardial infarction.