In the immunofluorescent analysis of γ-H2A.X foci, E2F1 enhancement modulated the TMZ-induced γ-H2A.X foci formation, which was elicited by the overexpression of miR-1258 in GBM cells (miR-1258+E2F1 vs. miR-1258+EV p < 0.01, Supplementary Figure S3B). The gene discussed is E2F1; the disease is glioblastoma.