To identify top 10 hub genes in CD14+ monocytes, DiseaseMeth version 2.0 analysis was performed, which showed significantly higher mean methylation levels for SIRT1, SKP2, TUBA1A, IMP3, EXOSC5, and SMAD4, while KRAS had significantly lower methylation level in samples from RA patients compared to healthy individuals (Figure 8C). The gene discussed is SMAD4; the disease is rheumatoid arthritis.