Correspondingly, cytoskeletal F-actin was reduced in LIMK1 knockdown Hs578T and PL-BC-05 cells, and increased in LIMK1-overexpressing MCF-7 cells, as verified by TRITC phalloidin staining, which showed clear changes in pseudopod and invadopodium formation in these cells (Fig. S4C). This evidence concerns the gene LIMK1 and breast cancer.