To expand our findings by evaluating the role of Lnc-408 and LIMK1 in metastasis, orthotopic xenografts were applied to nude mice by injecting engineered Hs578T cells and primary tumor cells (Hs578T/Control, Hs578T/sh Lnc-408, and Hs578T/sh Lnc-408/ectopic LIMK1; PL-BC-05/Control, PL-BC-05/KO Lnc-408, and PL-BC-05/KO Lnc-408/ectopic LIMK1) into the mammary fat pad. The gene discussed is LIMK1; the disease is neoplasm.