To identify how lncRNAs influence breast cancer invasion and metastasis, an RNA microarray was carried out to scan aberrant lncRNAs associated with tumor invasion and metastasis using Twist-induced EMT cells (MCF-7/Twist) and corresponding epithelial cells (MCF-7/Vec), as well as three BC tissues with or without lymphatic metastasis. This evidence concerns the gene TWIST1 and breast carcinoma.