Using AML-ETO, FLT3-ITD or MLL-ENL transfected cells after flow sorting to avoid cross-contamination with non-transfected progenitors, we observed that Mlkl−/− AML cells expressed significantly elevated levels of CSF3 mRNA (encoding for G-CSF) (Fig. 4B). This evidence concerns the gene RUNX1T1 and acute myeloid leukemia.