Despite no difference at 4 h postinfection, it was of note that the C358A Irgm2 mutant-reconstituted or empty vector-transduced Irgm2-deficient cells showed more rapid reduction in recruitment of p62 and ubiquitin at later time points (6 and 8 h post-infection) than wild-type Irgm2-reconstituted cells (Fig 5A and B). This evidence concerns the gene SQSTM1 and infection.