Furthermore, a number of signaling pathways previously implicated in different stages of NAFLD were also identified, including the signaling pathways of Wnt [38], insulin [49]; mTOR [26], TGF-β [50], NOD [51], TOLL-like receptor [52], VEGF [53], and PPARs [54]. This evidence concerns the gene INS and metabolic dysfunction-associated steatotic liver disease.