In addition, GSEA of methylation dynamic TSS loci in HPr1-AR cells strongly enriched for genes associated with cancer, including prostate cancer (e.g. WALLACE_PROSTATE_CANCER_RACE_UP) and specifically PCa with elevated H3K27me3 (KONDO_PROSTATE_CANCER_WITH_H3K27ME3) (Fig. 4C, Supplementary Fig. S3). This evidence concerns the gene AR and cancer.