We observed that miR-31-5p could be the better biomarker to predict cancer metastasis in clinics with AUC of 0.844, AUC of SATB2 + miR-31-5p group and SATB2 group is 0.835 and 0.767, respectively (Additional file 3: Fig. S3d). This evidence concerns the gene SATB2 and cancer.