Moreover, further studies based on the microarray assays of various human tumor samples demonstrated that the suppression of Nucb2 expression in the SW620 cells resulted in a low level of zinc finger E-box-binding homeobox transcription factor 1 (ZEB1), twist family bHLH transcription factor 1 (Twist), and snail family zinc finger 2 (Slug)—three transcription factors engaged in the EMT [19]. This evidence concerns the gene TWIST1 and neoplasm.