We examined the nucleotide distance between genetic positions of mutations across TP53 gene in ESCC tumor tissues and categorized them into three groups; less than 5 nucleotides distance between adjacent variants (15 variants), between 5 and 10 nucleotides (7 variants), and more than ten nucleotides distance between variants (22 variants). Here, TP53 is linked to esophageal squamous cell carcinoma.