Tumors treated with ginsenoside CK showed lower levels of hypoxia, as demonstrated by immunoblotting (Figure 8A) and immunohistochemistry staining (Figure 8B), implying that the accumulation and activity of HIF-1α in HCC-LM3 xenograft tumors can be regulated by treatment with ginsenoside CK. This evidence concerns the gene HIF1A and hepatocellular carcinoma.