PFKM and cancer: In comparison to the breast adipose tissue of women with benign tumors, malignant tumor-associated adipose tissue showed higher protein expression levels of AMPK and adipocyte-specific isoform of hexokinase—HK 2 (Figure 2A,B), while protein expression of downstream glycolytic enzymes PFK-1 and GAPDH did not change (Figure 2C,D).