Negatively regulated NF-κβ, PI3K/AKT, MAPK/JNK, and STAT pathways and inhibited MMP-7, MMP-9, and VEGF proteins are found to be key markers of the emodin.Like other cancer cell lines, emodin caused mitochondrial dysfunction and decreased mitochondrial membrane potential in colon cancer cell lines. This evidence concerns the gene NFKB1 and malignant colon neoplasm.