To test this possibility, we overexpressed RuvBL2 in Vero cells by transiently transfecting the cells with a plasmid that expressed RuvBL2 or empty vector for control, followed by infection of the transfected cells with WT or delNS1 PR8 virus at an MOI of 1 (mock infection for control) 24 h after the transfection. This evidence concerns the gene RUVBL2 and infection.