In contrast, a series of buttressed analogs of CDIM8 containing 3′- and 5′-substituents inhibited the growth, survival, and migration/invasion of ER-negative MDA-MB-231 and SKBR3 cells, and also mammary tumor growth in athymic nude mice bearing MDA-MB-231 cells (orthotopically) in the 2–5 mg/kg/d range of doses [21,22]. Here, ESR1 is linked to breast cancer.