Therefore, to validate our RNA-seq data of ANGPTL7, a qPCR analysis was performed using primary breast tumors of 4T1 and 4T1.2 compared with the expression level at the metastatic sites, e.g., spine, bone, and lung using 4T1.2 syngeneic recurrence/metastasis mouse model. The gene discussed is ANGPTL7; the disease is breast neoplasm.