FNDC5 and lung cancer: Nowinska et al. [80] found increased FNDC5 mRNA levels (measured by RT-PCR) and increased irisin protein levels (measured by Western blot and IHC) in human lung cancer cell lines NCI-1703 squamous cell carcinoma and NCI-H522 adenocarcinoma compared to IMR-90 healthy lung fibroblast cells.