A microarray-based multiplex autoantibody assay was developed, combining simultaneous detection of both APS-1-specific autoantibodies against type I IFNs and IL-22 and organ-specific autoantibodies against 21-OH, GAD-65, IA2, ICA, TG, and TPO. The gene discussed is PTPRN; the disease is autoimmune polyendocrine syndrome type 1.