In this study, a microarray-based multiplex assay was proposed for simultaneous detection of both organ-specific (anti-21-OH, anti-GAD-65, anti-IA2, anti-ICA, anti-Tg, and anti-TPO) and cytokine-specific (anti-IFN-ω, anti-IFN-α-2a, and anti-IL-22) autoantibodies that can be major biomarkers of autoimmune polyglandular syndromes. The gene discussed is TPO; the disease is autoimmune polyendocrinopathy.