The aim of this work was to develop and evaluate a multiplex method for detecting APS-specific autoantibodies, including autoantibodies (auto-Abs) to interleukin 22, omega and alpha-2-a interferons (IFN-ω and IFN-α-2a) and organ-specific autoantibodies to 21-OH, GAD-65, IA2, ICA, TG, and TPO. This evidence concerns the gene TPO and autoimmune polyendocrinopathy.