In addition, Tadano et al. [79] found that overexpression of hsa-miR-320e suppressed tumor proliferation, which may have been linked to decreased expression of CDK6. More recently, Wu et al. [80] demonstrated that forced expression of miR-320a/b/c/d/e inhibited cell proliferation and resulted in cell cycle arrest in osteosarcoma cells by targeting E2F transcription factor 1 (E2F1), a cell cycle regulator. The gene discussed is E2F1; the disease is osteosarcoma.