CD4 and neoplasm: The first significant result was that the activated CD4+ cells, inserted in t3D constructs, significantly increased the expression of CD69 compared to those embedded in the healthy matrix after 24 h of culture (p = 0.003), while CD25 expression was upregulated in the h3D compared to the tumor one, starting from the moment in which the lymphocytes were inserted in the 3D culture (0 h p = 0.010; 12 h p < 0.001; 24 h p < 0.003) (Figure 4a, right panels).