Alvala et al. [283] synthesized new imidazo [2,1-b]thiazole-sulfonyl piperazines (190a–f, Figure 71) and screened for their CA inhibitory activity against cytosolic (hCA I, hCA II) and the tumor-associated (hCA IX, hCAX II) isoforms using a stopped flow CO2 hydrase assay [269] and AAZ as a reference drug. The gene discussed is CYP24A1; the disease is neoplasm.