NES and glioma: To investigate whether inductive IR-resistant glioma cells in our experiments were related to autophagy and GSCs, we examined the expressions of P62 and LC3II as autophagy markers and CD133, CD44, and Nestin as GSC markers in two inductive IR-resistant glioma cell lines (GBM8401 and U87MG) irradiated with 2 Gy × 1 time dose and 2 Gy × 5 times dose.