GPC3 and hepatocellular carcinoma: We also assessed the expression level of glypican-3 (GPC3), a biomarker for diagnosis and prognosis of HCC [39], to address whether Pgrmc1 modulates HCC malignancy in vivo, and found that the GPC3 expression level in the tumor regions was comparable between WT and Pgrmc1-null livers (Figure S2).