GCLM and neoplasm: In order to verify whether SLC7A11, GCLM, and GLS are specifically induced by iron-loaded Lcn-2, we stimulated CAKI1 tumor cells with iron-free, apo-Lcn-2 (aLcn-2) and a mutant form (mLcn-2) deficient of its iron binding capacity compared to hLcn-2 (Figure 2A).